SNP-Based Cell Line Authentication
Reliable identification of human cell lines using advanced SNP profiling. A cost-effective alternative to conventional STR profiling, with additional capabilities for detecting cross-contaminating cell populations.
Reliable identification of human cell lines using advanced Single Nucleotide Polymorphism (SNP) profiling.
A cost-effective alternative to conventional STR profiling, with additional capabilities for cell line identification and contamination detection.
Order SNP Profiling →Our SNP-based approach provides reliable genetic authentication of human cell lines and offers several advantages over conventional STR profiling.
An affordable alternative to conventional STR-based cell line authentication.
Comparison against more than 960 distinct, STR-authenticated human reference cell lines. View database overview .
Robust authentication of cell lines carrying mutations in mismatch repair (MMR) genes, which can complicate conventional STR-based authentication.
Detection of minor contaminating cell populations down to approximately 10%, depending on the genetic relatedness of the cell lines.
Authentication of newly established human cell lines and sublines by comparing the genetic profiles of the original donor and derived cell line.
A comprehensive authentication report provides documented evidence of cell line identity for research documentation and publications.
Extract genomic DNA using commercially available DNA purification systems, for example from Qiagen or Roche.
Download DNA extraction protocol ↓
Complete our online order form and wait for the order confirmation email.
Quick Order Form →Once your samples arrive, Multiplexion takes care of the entire authentication process.
Genetic profiling of the submitted cell line.
Comparison of the resulting genetic signature against reference profiles.
Evaluation and interpretation of the authentication results.
Delivery of the authentication report by email.
The final report documents the authentication results and can be used to support grant applications and scientific publications.
Samples are processed during even-numbered calendar weeks. Results are usually delivered by email by the end of the respective processing week.
Samples can be dropped off at the DKFZ building INF242 at any time.
Please ensure that samples arrive by Friday before the scheduled processing week. Samples received during a given week are held and processed in the following scheduled testing week.
When reporting the use of our authentication service in grant applications or scientific publications, please use the following reference:
Reference for grant and paper submissions ↓If our analysis identifies a potentially contaminated or misidentified cell line, we recommend the following resources:
Recommendations on how to interpret and address contamination findings.
Read recommendations →Practical recommendations to help prevent cross-contamination in your laboratory.
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Reliable identification of human cell lines using advanced SNP profiling. A cost-effective alternative to conventional STR profiling, with additional capabilities for detecting cross-contaminating cell populations.